Inhibition of Cell Proliferation by DL-alpha-Difluoromethylornithine, a Catalytic Irreversible Inhibitor of Ornithine Decarboxylase.
نویسندگان
چکیده
منابع مشابه
Inhibition of human ornithine decarboxylase activity by enantiomers of difluoromethylornithine.
Racemic difluoromethylornithine (D/L-DFMO) is an inhibitor of ODC (ornithine decarboxylase), the first enzyme in eukaryotic polyamine biosynthesis. D/L-DFMO is an effective anti-parasitic agent and inhibitor of mammalian cell growth and development. Purified human ODC-catalysed ornithine decarboxylation is highly stereospecific. However, both DFMO enantiomers suppressed ODC activity in a time- ...
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The mechanism of inactivation of rodent ornithine decarboxylase by a-difluoromethylornithine (DFMO) was studied using the inhibitor labelled with 14C in both the 1 and the 5 positions. [1-14C]DFMO was a substrate and was decarboxylated by the enzyme yielding '4CO2. A radioactive metabolite derived from [5-'4C]DFMO was bound to the enzyme, and the extent of binding paralleled the irreversible in...
متن کاملAlpha-methyl ornithine, a potent competitive inhibitor of ornithine decarboxylase, blocks proliferation of rat hepatoma cells in culture.
A biphasic increase of putrescine concentration occurs in rat hepatoma tissue culture cells induced to proliferate. DL-alpha-Methyl ornithine, a competitive inhibitor of ornithine decarboxylase ( L-ornithine carboxylyase, EC 4.1.1.7) of hepatoma tissue culture cells, blocks the usual increases of putrescine and spermidine concentrations in these cells, and causes a rapid fall in the levels of p...
متن کاملInhibition of ornithine decarboxylase of HeLa cells by diamines and polyamines. Effect on cell proliferation.
1. Ornithine decarboxylase activity is stimulated in high-density HeLa-cell cultures by dilution of or replacement of spent culture medium with fresh medium containing 10% (v/v) horse serum. 2. After stimulation, ornithine decarboxylase activity reaches a peak at 4-6h, then rapidly declines to the low enzyme activity characteristic of quiescent cultures, where it remains during the remainder of...
متن کاملEffect on prostatic growth of 2-difluoromethylornithine, an effective inhibitor of ornithine decarboxylase.
2-Difluoromethylornithine (DFMO), an enzyme-activated irreversible inhibitor of ornithine decarboxylase, causes marked changes in the polyamine metabolism of ventral prostate when given to adult rats in drinking water (20 g/l) for 3 consecutive days. A 90% inhibition of ornithine decarboxylase activity is accompanied by approx. 80% decreases of the concentrations of putrescine and spermidine an...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1980
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.34b-0457